ABSTRACTS (BY NUMBER)

Tissue Engineering Part AVol. 28, No. S1 AbstractsFree AccessABSTRACTS (BY NUMBER)These are the abstracts as submitted through website. Last minute changes, title and presenting changes not always reflected in this file.Published Online:4 Apr 2022https://doi.org/10.1089/ten.tea.2022.29025.abstractsAboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail Abstract 1 ENABLING TECHNOLOGIES FOR DEVELOPMENT OF BIOARTIFICIAL ORGANSCorresponding author's email: [email protected] coming years, due aging of population low availability donor organs there will be urgent need for bioengineered assist, mimic or replace failing patient organs.These could artificial, based on novel biomaterials designs, assist a organ bioartificial: combining biologics (cells tissues) fully organs. In talk, we discuss enabling technologies development bioartificial with specific attention kidney, pancreas.References(1) “Innovations dialysis: Innovations dialysis membranes improved kidney replacement therapy”, Nature Reviews Nephrology, I. Geremia, D. Stamatialis (2020), 16(10) p550.2. “Frontiers hemodialysis: technological advances”, C Basile, A. Davenport, S. Mitra, Pal, Stamatialis, C. Chrysochou, Kirmizis, Artificial organs, 45 (2021) p175.Abstract 2 TRANSLATIONAL SYSTEMS BIOLOGY INFLAMMATION AND WOUND HEALING: FROM DATA TO MODELS DIGITAL TWINSCorresponding [email protected] regulated inflammation is central homeostasis, but adequate overly robust can lead disease. Like many biological processes, its various manifestations disease both multi‐dimensional multi‐compartmental. Our group has carried out studies aimed at obtaining high‐dimensional, dynamic data etiology progression inflammatory processes diseases samples derived from cells, animals, people; creating computational models these well key principles immunology, developing software tools aggregation analysis; modulating response an optimal spatial, temporal, individual‐ / disease‐specific manner. These center states, especially trauma/hemorrhage, infection, wound healing, liver failure, application approaches regenerative medicine, biomarker discovery, rational drug/device design. ultimate goal gain translational systems perspective (1,2).Keywords:Inflammation; Mathematical modeling; Systems biologyReferences1. An GV, Y. Translational Biology: Concepts Practice Future Biomedical Research. New York, NY: Elsevier; 2014.2. Complex Computational Biology Approaches Acute Inflammation: A Framework Model‐based Precision Medicine. Springer; 2020Abstract 3 RECENT ADVANCE TISSUE ENGINEERING ENHANCE NATURAL‐HEALING POTENTIALS REGENERATIVE MEDICINECorresponding [email protected]‐u.ac.jpA new therapeutic trial natural‐healing potential body itself induce tissues regeneration repairing, been recently expected. realize therapy, two cell transplantation tissue engineering. engineering biomaterial technology methodology artificially create local environment which enables cells enhance their proliferation differentiation regeneration. If scaffold bio‐signaling molecule supplied right place time period concentration, system initiates physiologically function, resulting natural induction cell‐based regeneration.The functions molecules promoted drug delivery (DDS) technology. Biodegradable hydrogels enabled controlled release growth factors chemokines succeed healing ‐based repairing recruitment activation cells. This and/or combined significantly efficacy The medicine also applicable basic researches stem biology. further biology effective enhancing regeneration.In paper, several applications DDS therapy research introduced emphasize clinical significance therapy.Keywords:tissue engineering; system; medicineAbstract 4 AUTOMATED PROCESS MANUFACTURING STRATEGIES CELL GENE THERAPIESCorresponding [email protected]‐modified cellular immunotherapies, including chimeric antigen receptor T‐cells (CAR‐T) killer (CAR‐NK), have generated significant commercial outcomes unparalleled rates against cancer. However, manufacture advanced therapies face number bottlenecks that must addressed ensure long‐term viability.We undertaken extensive investigate impact culture parameters yield quality CAR‐T medium screening studies, feeding strategies, dissolved oxygen concentrations, pH mode operation. outcome process control strategy resulted intensification efficiency >40% whilst maintaining critical attributes We established criteria scaling stirred‐tank bioreactors, ranging 15mL 1L, equivalent kinetic profiles.This talk introduce improve yield, outlining importance monitoring capability. role manufacturing discussed presented range expansion platforms. Finally, outline challenges scalable manufacture, particularly allogeneic (universal donor) applications, present 1L bioreactor studies.Keywords:ATMPs; Cell Therapy; Gene TherapyAbstract 5 DESIGN PHYSIOLOGICAL BIOREACTORS THOUGH IMAGE ANALYSIS COMPUTATIONAL MODELLINGCorresponding [email protected] biomanufacturing currently rely cultures suspensions inherently expensive, time‐consuming, variable comparison how products grown naturally. inefficiencies become human biomanufacturing, restricting future industrial translation lab‐grown Few deliver sufficient benefit, value, consistency offset high costs produce useful biomedical solutions. Recent advances image analysis modelling design operation high‐efficiency platforms, high‐content characterisation performance enable bioprocess control, optimisation, automation. aim maximize while minimizing variability expense. harness imaging image‐based design, fabricate, demonstrate efficient platforms.Keywords:Bioreactors; Imaging; BiofabricationAbstract 6 CRITICAL QUALITY ATTRIBUTES CARDIOVASCULAR CELLS DIFFERENTIATED HUMAN PLURIPOTENT STEM CELLSCorresponding [email protected] specialized types pluripotent (hPSCs) typically involves guiding developmentally‐relevant states via temporal presentation microenvironmental cues bioreactor. even precise over environment, prone line‐to‐line batch‐to‐batch because heterogeneity population. Failed inefficient runs contribute cost therapies. move toward closed loop real‐time integrated analytics inform composition state would presumably increase reproducibility. Here I efforts discover during hPSC cardiomyocytes multi‐omics populations extracellular media different stages differentiation. First, identified successful vs. failed differentiations, defined purity cTnT+ end differentiation, predicted early by metabolite profiling media. suggests differentiations stopped perhaps corrected interventions. metabolomic proteomic signatures correlate cardiomyocyte maturation state, achieved extended culture. results suggest metabolic pathways used identify potency metrics, may points vitro vivo applications.Keywords:Human Pluripotent Stem Cells; Cardiomyocytes; Critical Quality AttributesAbstract 7 SUPRAMOLECULAR HYDROGEL BIOMATERIALSCorresponding [email protected]–guest supramolecular recognition affords tool physical crosslinking macromeric precursors properties tunable applications. affinity certain macrocycle hosts binding suite guests offers rationally materials molecular scale so empower functionality. context use devices, modularity interactions facilitates opportunities combine multiple bioactivities payloads within single platform, routes facile incorporation targeting motifs functional complex milieu. subset macrocyclic host–guest chemistries able achieve affinities approaching biotin‐avidin, offering non‐covalent approach environments. Whereas conventional ligation reactions kinetically limiting, association occurs near diffusion limit. Accordingly, form quickly, considerable lifetime. work, describe our chemistry devices. protein modification means altering stability pharmacokinetics drugs. facilitate homing retention systemically administered small desired sites body, overcome dilution physiologic competition site‐specific accumulation.Abstract 9 3D PRINTABLE HYBRIDS ARTICULAR CARTILAGE REGENERATIONCorresponding [email protected] unmet help torn cartilage regenerate. Bouncy Bioglass (silica/polycaprolactone‐polytetrahydrofuran) hybrid [1] made coefficient friction matching articular (in media) [2]. Hybrids co‐networks inorganic organic components. gives unprecedented combination mechanical biodegradation. Hybrid inks were printed log‐pile‐like architectures responded cyclic loads [1]. found scaffolds guide bone marrow differentiated down chondrogenic route vitro, producing cartilage‐like matrix, rich Collagen II, Aggrecan GAG, only when pore channel size was 250 microns [3]. When larger, prevalent polycaprolactone where used, prevalent. Pore architecture, surface stiffness therefore affected response. sheep showed similar results, wherein micron pores excellent after months, defects remained 500 used.Keywords:hybrid; printing; differentiationReferences1. Tallia, F., Russo, L., Li, S., Orrin, A.L.H., Shi, X., Chen, Steele, J.A.M., Meille, Chevalier, J., Lee, P. D., Stevens, M. M., Cipolla, Jones, J. R., “, Materials Horizons, 2018, 5, 849‐860, DOI: 10.1039/C8MH00027A.2. Parkes, Young, G. Cann, P., Jeffers, R. T., Science Engineering: 2020: 119: 2021: 111495 doi.org/10.1016/j.msec.2020.111495.3. Mohammed, A., “Biomaterials Science, 8: 4458‐4466; 10.1039/C9BM01829H.Abstract 12 NOVEL CONCEPT MANUFACTURABILITY ITS IMPACT PRODUCTIONCorresponding KINO‐[email protected]‐U.AC.JPRegenerative transplants manufactured, requiring interdisciplinary activities medicinal fields field. ‘cell manufacturability’, capability bridging between aspects, proposed concept discipline production And “Design manufacturability” such way they easy simple, safe (cost‐saving) stable product secure customer considering transportation preparation outside factory, acting practically optimisation understanding requirement gap aspects reducing output fluctuation, action saving simplification governing kinetics behaviour.Based concepts back ground flexible modular plat [2], develop automation (machinery) retina epithelial pigment (RPE cells) iPS realise processing measuring motion. performed RPE compared experts laboratory. result concludes machinery manufacturing.Keywords:Cell manufacturability; Scale‐up Scale‐down; Automation systemReferences1. Kino‐oka M, Mizutani Medcalf N. Therapy Insights 1347, 2019;2. Kikuchi T, et al., Regenerative Therapy, 9, 89, 2018.Abstract 14 BIOFABRICATION: MOLECULAR CONTROL MACROSCOPIC FUNCTIONALITYAlvaro Mata11University NottinghamCorresponding [email protected] evolved grow regenerate using self‐assembling capable organizing wide variety building‐blocks scales. As field biofabrication progresses, it essential innovative ways capacity build more macroscopic structures nanoscale components organized across scales integrating chemistry, structural biology, hierarchical organization, heal, tuneable properties, selective bioactivity.Abstract 15 MESENCHYMAL CELL‐DERIVED EXTRACELLULAR VESICLES: EFFECTIVE MODULATORS THE IMMUNE SYSTEMBernd Giebel11Instute Transfusion Medicine, University Hospiatal EssenCorresponding bernd.giebel@uk‐essen.deExtracellular vesicles (EVs), harvested supernatant mesenchymal stem/stromal (MSCs) provide immune therapy. Compared therapeutics, EV‐based therapeutics advantages. Amongst others (70‐150 nm) sterilized filtration, lack any endogenous self‐replication much easier handle than Data demonstrating MSC‐EVs immunomodulatory properties. Furthermore, discussedAbstract 16 SKIN SUBSTITUTE WITH ARTIFICIAL DERMIS GRAFTED KERATINOCYTE SHEETS FULL‐THICKNESS BURN WOUNDSYosuke Niimi1,2, Suzan Alharbi2, Vsevolod L. Popov2, Hiroyuki Sakurai2, Dominique Wiener3, Donald Prough2, Perenlei Enkhbaatar21Tokyo women's medical university2University Texas Medical Branch3College Veterinary Medicine ScienceCorresponding [email protected]: study compare CEA overlaid excised full‐thickness burn wounds grafted either AD cadaver skin sheep.Methods: Six full thickness (5 × 5cm) induced dorsum sheep. After 24 hours, eschar fascia, covered ovine frozen (CS); AD. isolation autologous keratinocytes cultured temperature‐responsive dishes until forming multilayer sheets. Three weeks grafting, keratinocyte sheets onto CS epithelialization assessed days intermittent planimetric assay histological analysis.Results: Epidermis started rejecting 10 days, complete rejection observed 21 days. gradually changed dermis‐like tissue. dermis comparable groups 7, 14, 20 percentage epithelialized area sheet grafting (50.1 ± 7.8% vs 54.2 10.5%, p = 0.76) (91.2 4.1 87.4 5.8%, 0.60). transmission electron microscopy revealed no difference lamina densa hemidesmosomes groups.Conclusions: substitute grafts.Keywords:Keratinocyte sheet; temperature responsive dish; modelAbstract 17 TENDON STRUCTURE FUNCTION RELATIONSHIPS: INJURY INTERFASCICULAR MATRIXHazel Screen11Queen Mary, LondonCorresponding [email protected] injury debilitating recalcitrant. It primarily associated energy storing tendons, likely related greater demands placed tendons. mechanobiology tendon remains unclear.The focus specialisms storage, poor specialisation ageing turn increased risk.Our specialism focused non‐collagenous matrix fascicles, termed interfascicular (IFM). IFM elastin‐ lubricin‐rich, facilitating sliding recoil fascicles fatigue resistance [1‐3]. highly cellular, repaired maintained faster rate collagen fascicles. first region respond overload damage, suggesting initiate IFM.Tendons older, individuals show loss specialization reduced [4,5]. see turnover less renewal [6].Taken together, indicate active mechanoresponsive heavily loaded tendons health, leading injury.We now towards explore treatments injury, detail.References1. Thorpe CT, Udeze CP, Birch HL, Clegg PD, Screen HRC. Specialisation inter‐fascicular differences. J Roy Soc Int 9;76: 3108‐17; 2012.2. Karunaseelan KJ, Chieng Hin JN, Riley GP, Distribution proteins compartments varies according type. Anat. 229;3: 450‐8; 2016.3. Fascicles adaptation Acta Biomater. 15;42:308‐15; 2016.4. Capacity decreases equine tendons: mechanism age‐related tendinopathy? Eur Cells Mats 8;25:48‐60; 2013.5. decreased life Biomat. 56;58‐64. doi: 10.1016/j.actbio.2017.03.024; 2017.6. Peffers MJ, Collins JA, Eong R, Wei TK, HRC, PD. Proteomic reveals composition, age‐and injury‐specific fragmentation. Biol Chem. 289:37; 25867‐78; 2014.Abstract 18 CELL‐MATRIX INTERFACE ‐ UNDERSTANDING GUIDING FUNCTIONClaudia Loebel11University PennsylvaniaCorresponding [email protected] native microenvironment dynamic, synthesize, assemble, remodel surroundings development, repair. Biomaterials, hydrogels, valuable mechanisms cell‐extracellular (ECM) (e.g., mechanobiology) behavior repair/regeneration); however, nature cell‐ECM interface underappreciated. address this, utilizing labeling techniques visualize secreted better understand nascent influences designing proteolytically degradable viscoelastic cell‐hydrogel interactions. questions mechanosensing 3D(1), evolution modifying cartilage(2)), microfabricated hydrogel platforms organoids evolving cell‐material open up avenues biomaterials, allow us therapies.References1. Loebel, C., Mauck, Burdick, Local deposition remodelling stromal fate three‐dimensional hydrogels. 18, 883‐891, al. Metabolic Labeling Probe Spatiotemporal Accumulation Matrix Chondrocyte–Hydrogel Interface. Advanced Functional Materials, pressAbstract 19 SWEET SIDE BIOMATERIALS: CLARIFY ROLE GLYCANSFrancesco Nicotra1,21University Milano‐Bicocca2IUPACCorresponding [email protected] morphology biomolecular ECM induces fate, pathological events. Glycans indispensable ECMs, signals. interesting observe orchestrated homing, morphogenesis exploiting major classes biomolecules, glycans, synergistic quite roles. Structural features, swelling perform stronger weaker interactions, lower higher specificity, alternatively governed glycans versus proteins. Clarify contribution challenging dynamism surfaces. Evidences aging, diet, environmental pathologies influence glycosignature, cause‐effect relationship yet clarified. order spread light very intrigued matter, strategies needed generate ECMs functionalized responses. functionalization conjugate glycidic crosslink polysaccharide synthetic polymers described analyzed.ReferencesSampaolesi Nicotra Russo Nanomedicine, Impact Perspectives. Medicinal Chemistry, 11(1), 43, 2019Abstract ENGINEERED GRAFT STROMAL VASCULAR FRACTION ENCAPSULATED IN FIBRIN–COLLAGEN HYDROGEL: CLINICAL STUDY DIABETIC HEALINGMahsa Mollapour Sisakht1,21Skin Research Center, Tehran Sciences, Tehran, Iran2Department Biochemistry, Erasmus Rotterdam, NetherlandsCorresponding [email protected] abundance substitutes worldwide market, hurdles include addition appendages vascular networks most important structure. feasibility prevascularized grafts containing dermal epidermal layer adipose fraction (SVF)‐derived endothelial network Herein, characterized emphasis compatibility proliferation, migration, vitality. transplanted five subjects intervention diabetic nonvascularized patients. Wound biometric Mann–Whitney nonparametric t‐test (p ≤ .05). fibrin–collagen suitable organotypic There .05) density beds hypodermis measured scanner group. No differences summary, report dermal–epidermal intrinsic plexus study. preliminary SVF‐based accelerate process. next stage full‐scale randomized treatment patients chronic wounds.Keywords:skin fraction; healingReferencesNilforoushzadeh MA, Sisakht MM, Amirkhani Engineered graft encapsulated fibrin‐collagen hydrogel: healing. Eng Regen Med. 2020; 14(3):424‐440. doi:10.1002/term.3003.Abstract BIOMATERIALS MEDICINE INTERVERTEBRAL DISC: PARADIGM SHIFT SPINE SURGERYJerome Guicheux11INSERM U1229‐RMeS, Skeleton Centre, Nantes Hospital, ONIRIS, Nantes, France.Corresponding [email protected] disc (DDD), shares lot pathogenic (tissue degeneration, inflammation..) osteoarthritis (OA) one causes pain (LBP). Currently, LBP managed pharmacological if unsuccessful surgical procedures (spine fusion arthroplasty). clinically earlier degenerative cascade IVD, biology‐inspired r